Fig. 4

AChR dynamics is not altered in Mbnl1^ΔE3/ΔE3 and HSA^LR muscles. A Timeline of injections of α-bungarotoxin (Btx) for AChR turnover assay. d, days. Created with BioRender.com. B Turnover assay in EDL and TA muscles from Mbnl1^ΔE3/ΔE3 and HSA^LR mice. Fluorescent images show “old” (green) and “new” (red) AChRs in Mbnl1^ΔE3/ΔE3 and HSA^LR muscles. Scale bar, 50 μm. C, D AChR turnover in EDL and TA muscles from Mbnl1^ΔE3/ΔE3 (C) and HSA^LR (D) mice. n = 10/11 (EDL) and 5/5 (TA) Ctrl/Mbnl1^ΔE3/ΔE3 (C); 7/8 (EDL) and 3/3 (TA) Ctrl/HSA^LR (D), with more than 22 fibres per muscle. Data are mean ± SEM.