Fig. 3

Altered NMJ maintenance in Mbnl1^ΔE3/ΔE3 and HSA^LR mice. A, B Fluorescent images of NMJ regions stained with α-bungarotoxin (Btx, red), antibodies against neurofilament/synaptophysin (green), and dapi (blue) in EDL muscles from 3- and 9-month-old Mbnl1^ΔE3/ΔE3 mice (A) and 3-and 12-month-old HSA^LR mice (B). Scale bar, 50 μm. C, D Quantification of the number of fragments per endplate in EDL, TA and gastrocnemius (GA) muscles from 3- and 9/12-month-old Mbnl1^ΔE3/ΔE3 (C) and HSA^LR (D) mice. n = 5/8 (EDL 3 M), 3/4 (TA 3 M), 4/4 (GA 3 M), 5/3 (all muscles 9 M) Ctrl/ Mbnl1^ΔE3/ΔE3 (C); 11/12 (EDL 3 M), 7/8 (TA 3 M), 7/8 (GA 3 M), 4/3 (EDL 12 M), 4/4 (TA and GA 12 M) Ctrl/HSA^LR (D), with more than 50 fibres per muscle. Data represent mean ± SEM. **p < 0.01, ***p < 0.001, ****p < 0.0001, unpaired two-tailed Student’s t test