Fig. 2

CaMKIIβ deregulation in Mbnl1^ΔE3/ΔE3 and HSA^LR muscles. A-C Western blot analysis of CaMKII isoforms and quantification of CaMKIIβM levels in TA muscle from 3-month-old Mbnl1^ΔE3/ΔE3 mice (A) and in gastrocnemius muscle from 3-month-old HSA^LR mice (B). Quantification of CaMKIIβM phosphorylated form in Mbnl1^ΔE3/ΔE3 and HSA^LR muscles is shown in C. Protein levels are normalized on α-actinin (A, B) or on total CaMKIIβM (C), relative to control and expressed as log2(Fold Change). n = 3 (Ctrl/Mbnl1^ΔE3/ΔE3) and 4 (Ctrl/HSA^LR) per group. D-F Quantitative RT-PCR analysis of total Camk2b mRNA levels (D), Camk2 exon 13 exclusion (E), and Camk2b exons 18–20 inclusion (F) in TA muscle from Mbnl1^ΔE3/ΔE3 mice and in gastrocnemius (GA), TA and EDL muscles from HSA^LR mice. Data are normalized on Tbp levels (D) or on total Camk2b transcripts (E, F), relative to control and expressed as log2(Fold Change). n = 4 Ctrl / 3 Mbnl1^ΔE3/ΔE3; 4 Ctrl / 4 HSA^LR (D, E); 3 Ctrl / 3 HSA^LR (F). All data are mean ± SEM; * p < 0.05; ** p < 0.01; ***p < 0.001; **** p < 0.0001; two-tailed unpaired Student’s t-test