Fig. 6

A single cross between C57BL/6J and 129S1/SvlmJ increases IMAT formation providing a superior model for studying IMAT. A Breeding and experimental outline. B Body weight (g) of adult, 10-week-old C57BL/6J (Bl6), 129S1/SvlmJ and B6129SF1/J (B/129) males and females. Data are group to compare between: (Left) sex within the same strain; (Right) strains within the same sex. C (Top) Immunofluorescence of adipocytes (PERILIPIN⁺ cells; green) 21 days post injury (dpi) with Glycerol (GLY) in females (F) and males (M) from Bl6, B/129 & 129S mouse strains. Nuclei were visualized through DAPI (magenta). Scale bar: 500 µm. (Bottom) Muscle fibers were visualized through PHALLOIDIN and color-coded according to their cross-sectional area (CSA; um²). Scale bar: 250 µm. D Quantification of adipocytes normalized to injured area (adipocytes/mm²); and (E) average CSA normalized to body weight (µm²/g) 21 days post GLY injury in Bl6, 129S and B/129 female and male mice. B, D, E Data are grouped to compare between (Left) sexes within the same strain; (Right) strains within the same sex. B-E Data and images for Bl6 and 129S strains were taken from Fig. 1 for body weight, Fig. 2 for IMAT and Fig. 4 for muscle regeneration. F Breeding and experimental outline. G Body weight (g) of adult 10-week-old mixed C57BL/6J (Bl6_mix) and mixed N1 progeny (N1_mix) females and males. H Quantification of adipocytes normalized to injured area (adipocytes/mm²); I average cross-sectional area (CSA; µm²); and (J) average CSA normalized to body weight (µm²/g) 21 days post GLY injury of Bl6_mix and N1_mix of both sexes. All data are represented as mean ± SEM. An unpaired two-tailed t test or a one-way ANOVA followed by a Dunnet’s multiple comparison was used. A p value less than 0.05 was considered statistically significant where: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001 and **** p ≤ 0.0001