Fig. 3

Tissue fibrosis is mostly affected by strain and injury type but independent of sex. A Experimental outline. B Collagen deposition (red) of Tibialis Anterior (TA) cross sections was visualized by the histological stain Sirius Red 21 days post Cardiotoxin (CTX) or Glycerol (GLY) injury in C57BL/6J (Bl6), 129S1/SvlmJ (129S) & CD1 female (F) and male (M) mice. Scale bar: 500 µm. C-E Quantification of area occupied by collagen deposition normalized to total TA area (%). Data are grouped to compare between: C sexes within the same injury and strain; D strains within the same injury and sex; E injuries within the same sex and strain. F Summary model: Bl6 and CD1 strains are more fibrotic than 129S; and GLY induces a higher fibrotic response than a CTX model. All data are represented as mean ± SEM. An unpaired two-tailed t test or a one-way ANOVA followed by a Dunnet’s multiple comparison was used. A p value less than 0.05 was considered statistically significant where: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001 and **** p ≤ 0.0001