Fig. 2
IMAT infiltration post injury differs between strains, sexes, and injury types. A Experimental outline. B Immunofluorescence of mature adipocytes (PERILIPIN+ cells; green) 21 days post injury (dpi) with Cardiotoxin (CTX) or Glycerol (GLY), in females (F) and males (M) from C57BL/6J (Bl6), 129S1/SvlmJ (129S) & CD1 mouse strains. Nuclei are visualized through DAPI (magenta). Scale bar: 500 µm. C-E Quantification of adipocytes normalized to injured area (adipocytes/mm.2) 21 days after CTX or GLY injury in male (M) and female (F) Bl6, 129S & CD1 mice. Data are grouped to compare between: C sexes within the same injury and strain; D strains within the same injury and sex; E injuries within the same sex and strain. F Summary model: 129S and CD1 strains have more IMAT than Bl6; GLY induces more IMAT than CTX; and females have more IMAT than males. All data are represented as mean ± SEM. An unpaired two-tailed t test or a one-way ANOVA followed by a Dunnet’s multiple comparison was used. A p value less than 0.05 was considered statistically significant where: * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001 and **** p ≤ 0.0001