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Fig. 7 | Skeletal Muscle

Fig. 7

From: Cellular and molecular alterations to muscles and neuromuscular synapses in a mouse model of MEGF10-related myopathy

Fig. 7

Transcriptional analysis of Megf10 KO muscle. Bulk RNA-seq was performed on whole soleus muscles isolated from young adult (7 mo) female WT and Megf10 KO mice (n = 4), which (A) identified many upregulated and downregulated genes in Megf10 KO muscle. Differentially expressed genes in Megf10 KO muscle were related to (B) myogenesis, (C) muscle fiber type, (D) muscle stress, and (E) NMJ stress. Fold changes are displayed as relative expression in Megf10 KO muscle compared to WT muscle. (F) qPCR was used to confirm some of the differentially expressed genes identified by RNA-seq. (A-E) DESeq2 or (G) unpaired 2-sided Student’s t-test for statistical analysis. Values represented as mean + SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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