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Fig. 5 | Skeletal Muscle

Fig. 5

From: Cellular and molecular alterations to muscles and neuromuscular synapses in a mouse model of MEGF10-related myopathy

Fig. 5

Megf10 KO NMJs do not have disrupted PSC number or sprouting. (A) qPCR for Megf10 on FACS-isolated PSCs and other SCs from young adult (4–5 mo) female S100B-GFP; NG2-dsRed mice. (B) Representative images of NMJ postsynapses stained with fBTX (red), Schwann cells stained against S100B (green), presynapses and axons stained against SV2 and neurofilament (cyan), and postsynapses stained with DAPI (blue) from young adult (3 mo) male WT and Megf10 KO triangularis. PSCs are identified as S100B + Schwann cells overlying the NMJ postsynapse. These images were analyzed for (C) PSC number per NMJ, (D) the proportion of NMJs with a migrating Schwann cell, or (E) the proportion of NMJs in which PSCs extend sprouts away from the NMJ. Unpaired 2-sided Student’s t-test for statistical analysis. Values represented as mean + SD. **p < 0.01

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Skeletal Muscle

ISSN: 2044-5040

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