Fig. 2

Muscle fiber growth is decreased in Megf10 KO mice. (A) Representative images of tibialis anterior (TA) muscle cross sections from middle-aged female WT and Megf10 KO mice. TA cross sections from young adult and middle-aged female WT and Megf10 KO mice were examined for (B) fiber number per TA and (C) fiber size (minimum Feret diameter (MFD)). (D) Representative image of isolated TA muscles from middle-aged female WT and Megf10 KO mice. Whole muscle mass was recorded for the (E) TA and (F) soleus muscles. Two-way ANOVA with Tukey’s multiple comparison test for statistical analysis. Values represented as mean + SD. *p < 0.05, **p < 0.01, ****p < 0.0001 versus WT. ^†p < 0.05, ^††††p < 0.0001 versus young adult. YA (young adult, 3 mo), MA (middle-aged, 12–14 mo)