Fig. 3

Correlation between of eGFP and myofiber IIa and IIb in AAV9, AAVMYO, MyoAAV2A, MyoAAV4A-transduced muscles. Tibialis anterior muscle sections collected 5 weeks after injection in 6-week-old C57BL/6 mice with high dose (4.5E + 13 vg/kg) of AAVMYO-, MyoAAV2A-, MyoAAV4A- and AAV9-CMV-luc-IRES-eGFP. (A) Fluorescent co-labeling of eGFP, myofiber IIa and IIb in TA of AAVMYO-, MyoAAV2A-, MyoAAV4A- and AAV9-injected mice. Red arrowhead and yellow arrowhead indicate respectively GFP negative IIb fibers and GFP positive IIa fibers. Scale bar is 200 μm. (B) Quantification of positive GFP (GFP⁺) fibers depending of the fiber type. Percentage of GFP⁺ fibers was determined by the number of fibers presenting a colocalization between GFP and IIa/IIb signal divided by the total number of myofiber IIa/IIb. Data are presented as mean values +/- SEM (n = 3). Two-way ANOVA with Bonferroni correction