Fig. 6

PANX1 Overexpression Increases the Differentiation and Fusion of Human Dystrophic Myoblasts. A Dystrophic human skeletal muscle myoblasts (HSMM) (namely DMD 1, DMD 2, and DMD 7) were transduced with a lentivector containing either a GFP or PANX1 overexpression construct. Cells were differentiated for five days then stained for DAPI (blue), and MHC (red). Representative images shown are from DMD 1. Scale bar = 400 μm. HSMM were then analyzed for the B) differentiation (n = 3; two-tailed paired student’s t-test) and C) fusion index (n = 3; two-tailed paired student’s t-test). Each symbol on the graphs represents one cell line. Data represents mean ± s.d. * P < 0.05, ** P < 0.01, ns: non-significant. D Western blot of PANX1 in DMD cells (DMD 7) non-infected or infected with a lentivector containing GFP or PANX1 overexpression construct. GAPDH was used as a loading control