Fig. 4
The Tibialis Anterior Muscles of Panx1−/−/mdx mice have Fewer SCs. A Representative pictures of cross-sections of the TA muscles of Panx1−/−/mdx mice stained for DAPI (blue), necrotic fibers (IgG positive; green) and laminin (red) across the top and stained for DAPI (blue), Pax7 (green) and laminin (red) across the bottom. Scale bar = 100 μm. Cross-sections were then analyzed for the percentage of IgG positive fibers for the B) TA (n = 6; two-tailed unpaired student’s t-test) and C) Sol (n = 3–4; two-tailed unpaired student’s t-test). The number of SCs per mm2, identified by Pax7 staining, were measured in D) TA (n = 6; two-tailed unpaired student’s t-test) and E) Sol muscles (n = 4–6; two-tailed unpaired student’s t-test). Data represents mean ± s.d. ** P < 0.01, ns: non-significant