Fig. 3

Panx1^−/−/mdx mice TA Muscles are Smaller, Contain Less Myofibers with More that are Centrally Nucleated. A Representative images of the TA and Sol muscles of Panx1^−/−/mdx and Panx1^+/+/mdx cross-sections stained with H&E (top) and Masson trichrome (bottom). Scale bars = 200 μm. B The wet TA muscle weight was measured in both genotypes (n = 10; two-tailed unpaired student’s t-test). The TA C) mean CSA (n = 6; two-tailed unpaired student’s t-test), D) fiber CSA distribution (n = 6; two-way ANOVA followed by Sidak’s post hoc tests) and E) number of fibers per mm² (n = 5; unpaired two-tailed student’s t-test) were measured. The Sol F) wet muscle weight (n = 10; unpaired two-tailed student’s t-test), G) mean CSA (n = 6; unpaired two-tailed student’s t-test), H) fiber CSA distribution (n = 6; two-way ANOVA followed by Sidak’s post hoc tests) and I) number of fibers per mm² (n = 7–8; unpaired two-tailed student’s t-test) were also assessed. The percentage of fibers containing centrally located nuclei for J) the TA (n = 5; unpaired two-tailed student’s t-test) and K) the Sol (n = 6–7; two-tailed unpaired student’s t-test) were calculated. From the Masson trichrome staining the percentage of fibrotic area was calculated in the L) TA (n = 7; two-tailed unpaired student’s t-test) and the M) Sol (n = 5–7; two-tailed unpaired student’s t-test). Data represents mean ± s.d. * P < 0.05, ** P < 0.01, ns: non-significant