Fig. 6

Eldecalcitol reduces the expression of P65 and P52 and nuclear location of P65 in response to TNF-α. a–e Total protein levels of PP65, P65, and P52 were measured by Western blot analysis and nuclear protein level of P65. f Representative pictures of immunofluorescence showed that eldecalcitol reduced P65 nuclear translocation. Values are presented as mean ± SEM, n ≥ 3. *P < 0.05; **P < 0.01; ***P < 0.001 vs. control group; #P < 0.05; ##P < 0.01; ###P < 0.001 vs TNF-α group. Con, control; ELD, eldecalcitol; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; SEM, standard error of mean