Fig. 1

Single-cell RNA-sequencing identified Sox11 expression in differentiating muscle stem cells. A Phylogenetic neighbor-joining tree for the high-mobility group (HMG) domain containing Sox factors highlighting the SoxC subfamily and their domain phylogenetic similarity for mammals [44]. B UMAP plot of McKellar et al., aggregated dataset (GEO accession: GSE162172), broadly classified by cell type. C UMAP-based gene expression plots from McKellar dataset for Pax7, Myod1, Myog, and Sox11. D UMAP projection of scRNA-seq of MuSCs sorted from non-injured, 5 and 10 DPI muscle, colored by timepoint and cluster identity (left panel) (GEO accession: GSE150366). Violin plot of Pax7, Myog, and Sox11 based on scRNA-seq dataset to highlight expression in different MuSCs clusters. E qRT-PCR on RNA isolated from proliferating (D0) myoblasts and myoblasts induced to differentiate for 1, 2, and 3 days to detect the changes in expression of Myod1, myogenin, Sox11, and Sox4