Fig. 1

Flow chart for rapid membrane enrichment from mouse muscle. Skeletal muscle was fractionated as shown and described in detail in “Methods.” Lane numbers refer to analyses for total protein and immunoblotted sarcolemmal proteins, as shown in Fig. 2. Immunoprecipitations (IP, green ovals) with anti-Sgcg antibody were performed on extracts in either Rx buffer (high salt, 0.5% IGEPAL CA-630 in Rx buffer; lane 6) or with RIPA buffer (1% IGEPAL CA-630, 0.5% deoxycholate, 0.1% SDS; lane 8). WCL, whole cell lysate