Fig. 5

The effect of exercise on the expression of PGC1a/FNDC5/irisin. a Western blotting of PGC1a, FNDC5, and GAPDH was set as the loading control. n = 5 samples/group. b Quantification of PGC1a and FNDC5 protein levels. c Relative mRNA expressions of PGC1a and FNDC5 among groups. n = 5 samples/group. d Serum irisin levels of exercise and sedentary mice. n = 15 mice/group. *p < 0.05, **p < 0.01