Fig. 2

Gene expression and pathway analysis comparison between two MuSC subpopulations. a Volcano plot from comparing transcript levels between all cells within the “MuSC1” and “MuSC2” subpopulations. Log₂ fold-change in normalized gene expression versus −log₁₀ adjusted p value plotted. Differentially expressed genes (adjusted p value <0.05) are colored dark or light blue (based on their enrichment in MuSC1 or MuSC2, respectively). Genes with log₂ fold-change > 0.75 are labeled. b Normalized expression values of select differentially expressed genes. q values reported in inset. c Top activated canonical pathways by Ingenuity Pathway Analysis (IPA) based on differentially expressed genes and ranked by p value. Pathways significantly enriched in either population with |z score| > 1 are indicated in blue. d Select gene ontology (GO) terms and hallmark pathways enriched between the MuSC subpopulations as identified by gene set enrichment analysis (GSEA) and ranked by enrichment score (ES)