Fig. 2

Immunostaining of CD3+ (A.1–A.3), CD3−CD4+ (B.1–B.3), and CD11b+CD14+CD15+ (C.1–C.3) cells pointed by the white arrows in the muscle tissue of cancer patients. Stained nuclei in blue. A.1, B.1, and C.1 are the original images with no brightness manipulation. As for images A.2, B.2, and C.2, brightness was increased to visually appreciate the location of the immune cells on the periphery of muscle fibers. A.3, B.3, and C.3 were zoomed to a 400% from the original image. Scale bar 45 μm