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Fig. 3 | Skeletal Muscle

Fig. 3

From: Development of the excitation-contraction coupling machinery and its relation to myofibrillogenesis in human iPSC-derived skeletal myocytes

Fig. 3

Immunofluorescence detection of RyR1 and Cav1.1 channels in differentiating human iPSC-derived skeletal myocytes. A1–A4 Immunofluorescence labeling of RyR1 (red) and nuclei (blue) after 4 to 22 days of cell differentiation as indicated. B1–B4 Immunofluorescence labeling of Cav1.1 (green) and nuclei (blue); same time sequence after differentiation as in A1–A4, scale: 10 μm. C Frequency distribution histograms of RyR1 immunofluorescent particle area at early (7 days) or mature (22 days) stages of differentiation. Insets represent enlarged areas of RyRs immunolabeled cells to highlight the difference in size of the fluorescent particles between 7 and 22 days post-differentiation (scale: 10 µm). Data were obtained from three independent experiments, and immunofluorescent particles were counted from 13 and 8 cells in 7- and 22-day-old myocytes, respectively

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Skeletal Muscle

ISSN: 2044-5040

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