Fig. 7

IL-6 signaling pathway in the diaphragm and cardiac muscle. a Representative images of H&E and Masson’s trichrome staining of the diaphragm and heart of dKO mice treated with rat IgG or MR16-1. b Gene expression levels of Il-6, Il-6ra, Il-6se, and Socs3 in cardiac muscle were measured by real-time PCR. All samples were normalized using the gene expression levels of Tbp, and expressed as fold increase of wild-type mice (n = 3–4 per group). c The mRNA levels of genes associated with IL-6 (Il-6, Il-6ra, Il-6se, Socs3, and Stat3) were compared among the quadriceps muscle, diaphragm, and cardiac muscle of the same normal (non-treated) dKO mice at 90 days of age (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001