Fig. 2

Akt1 and Akt2 phosphorylation and total protein content in the gastrocnemius and soleus of bGH (n = 9) and WT (n = 9) male mice. Phosphorylation of Akt1 Serine 473 and Akt1 total protein in gastrocnemius of WT and bGH mice (a). Soleus phosphorylation of Akt1 Serine 473 and total protein in WT and bGH mice (b). Phosphorylation of Akt2 Serine 474 and Akt2 total protein in the gastrocnemius of bGH mice and WT controls (c). Soleus phosphorylation of Akt2 Serine 474 and total protein in the gastrocnemius of bGH mice and WT controls (d). Values in A.U. were normalized to a control sample run on each blot and then presented relative to GAPDH. Data are expressed as mean ± SEM. * P < 0.05, ** P < 0.005, ***P < 0.0005 vs. WT control mice. gastrocnemius, soleus