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Fig. 2 | Skeletal Muscle

Fig. 2

From: Expression patterns of FSHD-causing DUX4 and myogenic transcription factors PAX3 and PAX7 are spatially distinct in differentiating human stem cell cultures

Fig. 2

Human ES cells and iPS cell clones differentiate into skeletal myocytes with progressive expression of myogenic markers characteristic of early, middle, and late stages of myogenesis. a Schematic of the differentiation protocol. C = CHIRON99021, L = LDN193189, F = FGF2, H = HGF, I = IGF, N2 = neuronal supplement. b–d Images of isogenic clone hiPSC-mosaic1 with the long D4Z4 array. e–g Images of hiPSC-mosaic 1 with the short D4Z4 array. b, e Bright field images of cells at the various stages shown in a. Cell morphology proceeds from small stem-like cells (D7) to spindle-shaped elongated cells more characteristic of myoblasts (D30) to multinucleate elongated fibers on D40. c, f Immunofluorescence images of PAX3-stained cells early in the protocol (D7) that progress to differentiated myocytes with d, g immunoreactivity for PAX7and Titin. Insets show magnified view of cells within the white boxes

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Skeletal Muscle

ISSN: 2044-5040

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