Fig. 4

Myofiber branching is decreased in mdx muscles over-expressing mOR23. a Representative images of 8-week-old mdx and Tg-mdx gastrocnemius muscles. Bar = 100 μm. b The percentage of regenerated myofibers was not significantly different between mdx and Tg-mdx muscles at the three ages (5, 8, and 15 weeks) studied. c Tg-mdx muscles had significantly less branched regenerated myofibers than mdx muscles at 5 and 8 weeks of age. d The number of branches per branched myofiber was also significantly decreased in Tg-mdx muscles compared to mdx at both 8 weeks (chi-square = 12.3, df = 3) and 15 weeks of age (chi-square = 13.9, df = 3). n = 32–103 myofibers isolated per age and mouse. Data are mean ± SEM and n = 3–4 for each genotype at each age with *p < 0.05