Figure 7

Over-expression of PGC-1α results in enhanced lysosomal protein expression induced by denervation. (A) Autophagy gene expression. mRNA fold changes between wild type (WT) and PGC-1α Tg (Tg) control (Con) and denervated (Den). All groups are compared to WT con, Gapdh and Actb were used as housekeeping genes. (B-G) Blots and quantification of autophagic proteins in Con, Den WT and Tg animals. (B) Representative blots. Quantification of (C) Parkin, (D) Beclin 1, (E) Atg7, (F) Cathepsin D, and (G) Lamp-2. *P < 0.05 significant difference between Con and Den. †P < 0.05, significant effect of genotype. GAPDH was used as a loading control (n = 3 to 5 for all groups). AU, arbitrary units; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.