Figure 1

MEFs transduced with MyoD lentivirus. (A) MEFs transduced with MyoD lentivirus demonstrate nearly complete conversion into myotubes 72 h after infection (red: MYOD antibody; green: myosin heavy chain antibody; blue: DAPI). (B) Western blot demonstrates higher MyoD expression in MEFs transduced with MyoD lentivirus (2) compared to control MEFs (1), C2C12 myoblasts (3), and C2C12 myotubes (4). Tubulin blot demonstrates equivalent loading.