Figure 3
From: Skeletal muscle proteomics: current approaches, technical challenges and emerging techniques
Overview of the fluorescence difference in-gel electrophoretic (DIGE) method. Shown is a diagram of the differential labeling of muscle specimens with the fluorescent CyDyes Cy2, Cy3 and Cy5, as well as an example of a DIGE analysis of myosin light chain isoforms during fast-to-slow transitions of electrostimulated skeletal muscle.